Anticorps Monoclonal anti-PDE6D

PDE6D Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1H4B6

Avis (2)

N° de cat : 67565-1-Ig

Synonymes

GMP PDE delta, PDE6D, PDED, Protein p17

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Galerie de données de validation

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  WB analysis of Jurkat using 67565-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67565-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67565-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67565-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67565-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat retina using 67565-1-Ig

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67565-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HepG2, cellules JAR, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu placentaire humain, tissu rétinien de rat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67565-1-Ig cible PDE6D dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDE6D Protéine recombinante Ag29563
• Nom complet: phosphodiesterase 6D, cGMP-specific, rod, delta
• Masse moléculaire calculée: 17 KDa
• Poids moléculaire observé: 15-17 kDa
• Numéro d’acquisition GenBank: NM_002601
• Symbole du gène: PDE6D
• Identification du gène (NCBI): 5147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Charlotte (Verified Customer) (07-24-2023)

We tested the antibody against PDE6 delta KO cells. We can see the antibody is very noisy but it is working.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: NIH-3T3

PDE6D Antibody Western Blot validation (1/2000 dilution) in NIH-3T3 (Cat no:67565-1-Ig)

FH

Criseyda (Verified Customer) (09-19-2022)

The antibody had background bands/nonspecific bands present but worked best in 5% Milk TBS-T (Blocking, Primary and Secondary) and not 3% BSA TBS-T (Blocking, Primary and Secondary).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: T47D

PDE6D Antibody Western Blot validation (1:1000 dilution) in T47D (Cat no:67565-1-Ig)