Anticorps Monoclonal anti-PDE4D

PDE4D Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1C4B9

Avis (1)

N° de cat : 67062-1-Ig

Synonymes

DPDE3, HSPDE4D, PDE43, PDE4D, PDE4DN2, STRK1

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Galerie de données de validation

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  WB analysis using 67062-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67062-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67062-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67062-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67062-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de porc, tissu de cervelet de rat, tissu de cervelet de souris

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67062-1-Ig cible PDE4D dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDE4D Protéine recombinante Ag8302
• Nom complet: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Masse moléculaire calculée: 809 aa, 91 kDa
• Poids moléculaire observé: 91-120 kDa, 66-84 kDa
• Numéro d’acquisition GenBank: BC036319
• Symbole du gène: PDE4D
• Identification du gène (NCBI): 5144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cancer Med; Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma; Authors - Yimin Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angie (Verified Customer) (06-06-2023)

Protein lysates from A549 cells were subjected to SDS-PAGE followed by immunoblotting with 67062-1-Ig (mouse anti-PDE4D antibody) at dilution of 1:10 000 incubated overnight at 4°C. Secondary antibody: Donkey-anti-mouse (Alexa Fluor 680) at 1:20000 dilution incubated at room temperature for 1 hour.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10 000
• Cell Tissue Type: Human lung cancer cell A549

PDE4D Antibody Western Blot validation (1:10 000 dilution) in Human lung cancer cell A549 (Cat no:67062-1-Ig)