Anticorps Polyclonal de lapin anti-PDE2A

• Phare
• Validé par KD/KO

PDE2A Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 55306-1-AP

Synonymes

CGS PDE, cGSPDE, PDE2A, PDE2A1, PED2A4

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Galerie de données de validation

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  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55306-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55306-1-AP

  IP Result of anti-PDE2A (IP:55306-1-AP, 4ug; Detection:55306-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

55306-1-AP cible PDE2A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phosphodiesterase 2A, cGMP-stimulated
• Masse moléculaire calculée: 106 kDa
• Poids moléculaire observé: 100-106 kDa
• Numéro d’acquisition GenBank: NM_002599
• Symbole du gène: PDE2A
• Identification du gène (NCBI): 5138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE2A, also named as cGSPDE and CGS-PDE, belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. It is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. This antibody is specific to PDE2A.

Publications

Species	Application	Title
mouse	WB	Sci Adv; RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.; Authors - Mehdi Eshraghi
rat	WB	Br J Pharmacol; 17β-estradiol reduces mitochondrial cAMP content and cytochrome oxidase activity in a phosphodiesterase 2-dependent manner.; Authors - Sofya Pozdniakova
rat	WB	Biochem Pharmacol; Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A.; Authors - Módis Katalin K
rat	WB	Front Pharmacol; Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress.; Authors - Xiaofei Zhu
human,mouse	WB,IF	Commun Biol; Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy.; Authors - Miguel J Lobo ; KO Validated
rat	WB	JCI Insight; Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity.; Authors - Kun Liu