Anticorps Monoclonal anti-CCM3/PDCD10

• Phare
• Validé par KD/KO

CCM3/PDCD10 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC

Conjugaison

Non conjugué

CloneNo.

1E5A8

N° de cat : 66440-1-Ig

Synonymes

CCM3, PDCD10, programmed cell death 10, TFAR15

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Galerie de données de validation

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  WB analysis of U-251 using 66440-1-Ig

  WB result of CCM3/PDCD10 antibody (66440-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDCD10 transfected U-251 cells.

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  WB analysis of Jurkat using 66440-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66440-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 66440-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66440-1-Ig (CCM3/PDCD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66440-1-Ig (PDCD10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66440-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66440-1-Ig (CCM3/PDCD10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Ramos, cellules U-251, plaquettes de sang périphérique humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66440-1-Ig cible CCM3/PDCD10 dans les applications de WB, IHC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCM3/PDCD10 Protéine recombinante Ag0348
• Nom complet: programmed cell death 10
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC002506
• Symbole du gène: PDCD10
• Identification du gène (NCBI): 11235
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDCD10, also named CCM3 and TFAR15, belongs to the PDCD10 family. PDCD10 promotes cell proliferation, increases MAPK activity and modulates apoptotic pathways. PDCD10 is important for cell migration and for normal structure and assembly of the Golgi complex. PDCD10 is required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development, moreover, defects in PDCD10 showed abnormal cardiovascular development.