Anticorps Monoclonal anti-PD-L1 (B7-H1)

PD-L1 (B7-H1) Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

29E.2A3

N° de cat : CL488-65081

Synonymes

B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1 (B7-H1), Programmed death ligand 1

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Galerie de données de validation

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  FC experiment of human PBMCs using CL488-65081

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human PD-L1 (B7-H1) (CL488-65081, Clone: 29E.2A3) (red) or Mouse IgG2b Isotype Control (blue). Unactivated PBMCs were surface stained with CL488-65081 (black). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL488-65081

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human PD-L1 (B7-H1) (CL488-65081, Clone: 29E.2A3) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65081 cible PD-L1 (B7-H1) dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PD-L1 humain pleine longueur
• Nom complet: CD274 molecule
• Numéro d’acquisition GenBank: BC069381
• Symbole du gène: CD274
• Identification du gène (NCBI): 29126
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.