Anticorps Monoclonal anti-PD-1/CD279
PD-1/CD279 Monoclonal Antibody for FC, IF
Hôte / Isotype
Mouse / IgG2b
Réactivité testée
Humain, rat, souris
Applications
IF, FC
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
4H4D1
N° de cat : CL488-66220
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IF | tissu d'amygdalite humain, |
Résultats positifs en cytométrie | CD3 antibody treated Jurkat cells |
Dilution recommandée
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL488-66220 cible PD-1/CD279 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Hôte / Isotype | Mouse / IgG2b |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | PD-1/CD279 Protéine recombinante Ag12470 |
Nom complet | programmed cell death 1 |
Masse moléculaire calculée | 288 aa, 32 kDa |
Numéro d’acquisition GenBank | BC074740 |
Symbole du gène | PDCD1 |
Identification du gène (NCBI) | 5133 |
Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Forme | Liquide |
Méthode de purification | Purification par protéine A |
Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438). This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
Protocole
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 PD-1/CD279 antibody CL488-66220 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |