Anticorps Polyclonal de lapin anti-PD-1/CD279
PD-1/CD279 Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, ELISA
Conjugaison
Non conjugué
N° de cat : 29780-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu de thymus de souris, tissu de thymus de rat |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
29780-1-AP cible PD-1/CD279 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | PD-1/CD279 Protéine recombinante Ag31288 |
Nom complet | programmed cell death 1 |
Masse moléculaire calculée | 288 aa, 32 kDa |
Poids moléculaire observé | 55 kDa |
Numéro d’acquisition GenBank | BC074740 |
Symbole du gène | PDCD1 |
Identification du gène (NCBI) | 5133 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).
Protocole
Product Specific Protocols | |
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WB protocol for PD-1/CD279 antibody 29780-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |