Anticorps Polyclonal de lapin anti-PARP2

PARP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 55149-1-AP

Synonymes

ADPRT2, ADPRTL3, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.-

---

Galerie de données de validation

• 

  WB analysis using 55149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55149-1-AP (PARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 55149-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55149-1-AP (PARP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SH-SY5Y, cellules U2OS
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below

Informations sur le produit

55149-1-AP cible PARP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: poly (ADP-ribose) polymerase 2
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: NM_005484
• Symbole du gène: PARP2
• Identification du gène (NCBI): 10038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP2, also named as ADPRT2 and ADPRTL2, is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. This antibody is specific to PARP2 (N-terminal).

Publications

Species	Application	Title
human	WB	Oncogene; CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma.; Authors - Xueying Wang
	WB	Cells; Poly(ADP-Ribose) Polymerase-1 Lacking Enzymatic Activity Is Not Compatible with Mouse Development; Authors - Tatiana Kamaletdinova
human	WB	Eur J Med Chem; Selective degradation of PARP2 by PROTACs via recruiting DCAF16 for triple-negative breast cancer.; Authors - Chunlan Pu
mouse	WB	Int J Biol Sci; PARP1 Promotes Heart Regeneration and Cardiomyocyte Proliferation; Authors - Jiangcheng Shu
rat,mouse	WB	EMBO J; Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function; Authors - Quanjiang Zhang
human	WB	Eur J Med Chem; Leveraging a rationally designed veliparib-based anilide eliciting anti-leukemic effects for the design of pH-responsive polymer nanoformulation; Authors - Amandeep Thakur

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hasan (Verified Customer) (12-07-2022)

WB analysis for U2OS cells (WT and PARP2KO) with PARP2 Ab (55149-1-AP) at dilution of 1:2000 overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

PARP2 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:55149-1-AP)

{{ptg:RelatedPrimaryAntibodies}}