Anticorps Monoclonal anti-PARP1

PARP1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1D7D4

N° de cat : CL594-66520

Synonymes

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

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Galerie de données de validation

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  IF Staining of HeLa using CL594-66520

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66520 (PARP1 antibody) at dilution of 1:100.

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  IF Staining of Neuro-2a using CL594-66520

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CoraLite®594 PARP1 antibody (CL594-66520, Clone: 1D7D4 ) at dilution of 1:2000, CL488-Phalloidin (green).

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  FC experiment of HeLa using CL594-66520

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PARP1 (CL594-66520, Clone:1D7D4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of Jurkat using CL594-66520

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human PARP1 (CL594-66520, Clone:1D7D4) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules HeLa, cellules Neuro-2a
• Résultats positifs en cytométrie: cellules HeLa, cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66520 cible PARP1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PARP1 Protéine recombinante Ag19173
• Nom complet: poly (ADP-ribose) polymerase 1
• Masse moléculaire calculée: 1014 aa, 113 kDa
• Poids moléculaire observé: 113-116 kDa, 85-89 kDa
• Numéro d’acquisition GenBank: BC037545
• Symbole du gène: PARP1
• Identification du gène (NCBI): 142
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kD. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011