Anticorps Monoclonal anti-PAI-1

PAI-1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

CloneNo.

1H4A5

Avis (1)

N° de cat : 66261-1-Ig

Synonymes

PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1

---

Galerie de données de validation

• 

  WB analysis of human peripheral blood platelets using 66261-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66261-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig serum using 66261-1-Ig

  pig serum were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 66261-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 66261-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 66261-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66261-1-Ig (PAI-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66261-1-Ig (PAI-1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse lung using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 66261-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66261-1-Ig (PAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66261-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAI-1 antibody (66261-1-Ig, Clone: 1H4A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain, cellules HuH-7, cellules SH-SY5Y, cellules U-251, tissu hépatique de porc
• Résultats positifs en IHC: tissu rénal humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66261-1-Ig cible PAI-1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAI-1 Protéine recombinante Ag22411
• Nom complet: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Masse moléculaire calculée: 402 aa, 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC010860
• Symbole du gène: SERPINE1
• Identification du gène (NCBI): 5054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Identification of Impacted Pathways and Transcriptomic Markers as Potential Mediators of Pulmonary Fibrosis in Transgenic Mice Expressing Human IGFBP5.; Authors - Xinh-Xinh Nguyen
human	WB	Am J Respir Cell Mol Biol; ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial Plasminogen Activator Inhibitor-1.; Authors - Mingming Han
mouse	WB	Clin Sci (Lond); Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability.; Authors - Mengqiu Wu
human	WB	Phytomedicine; Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3.; Authors - Tong Lin
human	IHC	Cancer Biomark; Weighted correlation network analysis identifies FN1, COL1A1 and SERPINE1 associated with the progression and prognosis of gastric cancer; Authors - Qiaoyun Zhao
mouse	WB,IHC	Arch Biochem Biophys; Human umbilical cord mesenchymal stem cell-derived exosomal microRNA-148a-3p inhibits neointimal hyperplasia by targeting Serpine1.; Authors - Xiaoyu Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Mona (Verified Customer) (10-17-2021)

Paraffin-embedded hamster tissue from the lung. Antigen retrieval was performed with Tris-EDTA buffer (pH 9).

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: hamster (lung)