Anticorps Monoclonal anti-PABPN1

• Phare
• Validé par KD/KO

PABPN1 Monoclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

3C12E12

N° de cat : 66807-1-Ig

Synonymes

OPMD, PAB2, PABII, PABP 2, PABP2, PABPN1, Poly(A) binding protein 2, Poly(A) binding protein II

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Galerie de données de validation

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  WB analysis using 66807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66807-1-Ig

  WB result of PABPN1 antibody (66807-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPN1 transfected HeLa cells.

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  WB analysis using 66807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 66807-1-Ig

  IP result of anti-PABPN1 (IP:66807-1-Ig, 5ug; Detection:66807-1-Ig 1:600) with Jurkat cells lysate 1680 ug.

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  IHC staining of human colon using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66807-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque de rat, tissu cardiaque de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

66807-1-Ig cible PABPN1 dans les applications de WB, RIP, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PABPN1 Protéine recombinante Ag27470
• Nom complet: poly(A) binding protein, nuclear 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 50-56 kDa
• Numéro d’acquisition GenBank: BC010939
• Symbole du gène: PABPN1
• Identification du gène (NCBI): 8106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).

Publications

Species	Application	Title
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
mouse	WB	J Biol Chem; Extracellular 5'-methylthioadenosine inhibits intracellular symmetric dimethylarginine protein methylation of FUSE-element binding proteins; Authors - Baiqing Tang
human	WB,IHC,RIP	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen ; KD Validated
human	WB	Nat Cell Biol; An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus; Authors - Albertas Navickas