Anticorps Polyclonal de lapin anti-P2RX7

• Phare
• Validé par KD/KO

P2RX7 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 28207-1-AP

Synonymes

Homo sapiens (Human), P2RX7, P2X7

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Galerie de données de validation

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  WB analysis using 28207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28207-1-AP (P2RX7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de cervelet de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Informations sur le produit

28207-1-AP cible P2RX7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P2RX7 Protéine recombinante Ag27700
• Nom complet: purinergic receptor P2X, ligand-gated ion channel, 7
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC011913
• Symbole du gène: P2RX7
• Identification du gène (NCBI): 5027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Publications

Species	Application	Title
mouse,rat	WB	J Cell Physiol; Apelin-13/APJ induces cardiomyocyte hypertrophy by activating the Pannexin-1/P2X7 axis and FAM134B-dependent reticulophagy.; Authors - Yiyuan Yang ; KD Validated
mouse,rat	WB,IHC,IF	Front Pharmacol; Salidroside Ameliorates Depression by Suppressing NLRP3-Mediated Pyroptosis via P2X7/NF-κB/NLRP3 Signaling Pathway.; Authors - Yuhui Chai
human	WB	Biochem Biophys Res Commun; A438079 affects colorectal cancer cell proliferation, migration, apoptosis, and pyroptosis by inhibiting the P2X7 receptor.; Authors - Ying Zhang
human	WB,IHC	Adv Med Sci; The role of P2X7 receptor in prognosis and metastasis of colorectal cancer.; Authors - Ying Zhang
human	WB	Biomed Res Int; Progesterone Reduces ATP-Induced Pyroptosis of SH-SY5Y Cells; Authors - Chang Cui
human,mouse	WB,IHC	Phytother Res; Asiaticoside hampers epithelial-mesenchymal transition by promoting PPARG expression and suppressing P2RX7-mediated TGF-β/Smad signaling in triple-negative breast cancer; Authors - Xuemei Huang