Anticorps Monoclonal anti-P27; KIP1

P27; KIP1 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB

Conjugaison

Non conjugué

Publications

1

CloneNo.

3F12C10

N° de cat : 67355-1-Ig

Synonymes

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27KIP1

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Galerie de données de validation

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  WB analysis of Jurkat using 67355-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67355-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67355-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67355-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HSC-T6, cellules K-562, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67355-1-Ig cible P27; KIP1 dans les applications de WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: P27; KIP1 Protéine recombinante Ag14634
• Nom complet: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• Masse moléculaire calculée: 198 aa, 22 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC001971
• Symbole du gène: CDKN1B
• Identification du gène (NCBI): 1027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Publications

Species	Application	Title
human	WB	J Hepatocell Carcinoma; CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27 kip1 Axis; Authors - Xin Zhou