Anticorps Polyclonal de lapin anti-P27; KIP1

• Phare
• Validé par KD/KO

P27; KIP1 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 26714-1-AP

Synonymes

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 26714-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26714-1-AP

  WB result of P27; KIP1 antibody (26714-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P27; KIP1 transfected HeLa cells.

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  WB analysis of MCF-7 using 26714-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26714-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26714-1-AP (P27; KIP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 26714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 26714-1-AP (P27; KIP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 26714-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human P27; KIP1 (26714-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu de gliome humain, tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

26714-1-AP cible P27; KIP1 dans les applications de WB, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P27; KIP1 Protéine recombinante Ag25083
• Nom complet: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• Masse moléculaire calculée: 198 aa, 22 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC001971
• Symbole du gène: CDKN1B
• Identification du gène (NCBI): 1027
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Publications

Species	Application	Title
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	WB	Biosci Rep; C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo.; Authors - Wei Zhang

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011