Anticorps Polyclonal de lapin anti-P21

P21 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

23

N° de cat : 27296-1-AP

Synonymes

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, PIC1, SDI1, WAF1

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Galerie de données de validation

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  WB analysis using 27296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27296-1-AP (P21 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 27296-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (27296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules C6, cellules NIH/3T3
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

27296-1-AP cible P21 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P21 Protéine recombinante Ag26129
• Nom complet: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000275
• Symbole du gène: CDKN1A
• Identification du gène (NCBI): 1026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publications

Species	Application	Title
human	WB,IF	Am J Respir Crit Care Med; CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging; Authors - Huachun Cui
mouse	WB	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
mouse	WB	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
mouse	IF	Cell Mol Life Sci; Silencing circSERPINE2 restrains mesenchymal stem cell senescence via the YBX3/PCNA/p21 axis; Authors - Fenglei Chen
mouse	WB	Oxid Med Cell Longev; PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.; Authors - Yanzhuo Zhang
mouse	IF	Front Cell Dev Biol; P53 independent pathogenic mechanisms contribute to BubR1 microcephaly; Authors - Noelle A Sterling