Anticorps Polyclonal de lapin anti-Osteopontin

Osteopontin Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 30200-1-AP

Synonymes

AA960535, AI790405, Apl 1, BNSP, Bsp, BSPI, Eta, ETA 1, minopontin, OP, Opn, Opnl, Osteopontin, Ric, secreted phosphoprotein 1, Spp 1, Spp1

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Galerie de données de validation

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  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30200-1-AP (Osteopontin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of RAW 264.7 using 30200-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Osteopontin antibody (30200-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, tissu rénal de rat, tissu rénal humain
• Résultats positifs en IF: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30200-1-AP cible Osteopontin dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: secreted phosphoprotein 1
• Masse moléculaire calculée: 32kd
• Poids moléculaire observé: 32 kDa, 60-70 kDa
• Numéro d’acquisition GenBank: NM_001204233.1
• Symbole du gène: Spp1
• Identification du gène (NCBI): 20750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594). Osteopontin (OPN) is an acidic 60-70 kDa glycoprotein that is cleaved by proteases to yield 45-kDa and 24-kDa fragments (PMID: 10491621, PMID: 1995615)