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Anticorps Polyclonal de lapin anti-Occludin

Occludin Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-27260

Synonymes

occludin, OCLN

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated Occludin Polyclonal (CL488-27260)

    IF Staining of MCF-7 using CL488-27260

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:800.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CoraLite® Plus 488-conjugated Occludin Polyclonal (CL488-27260)

    IF Staining of Caco-2 using CL488-27260

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 Occludin antibody (CL488-27260) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated Occludin Polyclonal (CL488-27260)

    FC experiment of MCF-7 using CL488-27260

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Occludin (CL488-27260) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules MCF-7, cellules Caco-2
Résultats positifs en cytométriecellules MCF-7

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-27260 cible Occludin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Occludin Protéine recombinante Ag26173
Nom complet occludin
Masse moléculaire calculée 522 aa, 59 kDa
Numéro d’acquisition GenBankBC029886
Symbole du gène OCLN
Identification du gène (NCBI) 4950
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 Occludin antibody CL488-27260Download protocol
FC protocol for CL Plus 488 Occludin antibody CL488-27260Download protocol
Standard Protocols
Click here to view our Standard Protocols