Anticorps Monoclonal anti-OXCT1

• Phare
• Validé par KD/KO

OXCT1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G1B9

N° de cat : 67836-1-Ig

Synonymes

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

---

Galerie de données de validation

• 

  WB analysis of rat heart using 67836-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67836-1-Ig

  WB result of OXCT1 antibody (67836-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

• 

  WB analysis of HeLa using 67836-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67836-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67836-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67836-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67836-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 67836-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67836-1-Ig (SCOT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 67836-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67836-1-Ig (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 67836-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 67836-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 67836-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (67836-1-Ig, Clone: 1G1B9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 67836-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OXCT1 (67836-1-Ig, Clone:1G1B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, cellules HeLa, cellules Jurkat, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67836-1-Ig cible OXCT1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OXCT1 Protéine recombinante Ag24792
• Nom complet: 3-oxoacid CoA transferase 1
• Masse moléculaire calculée: 520 aa, 56 kDa
• Poids moléculaire observé: 52-56 kDa
• Numéro d’acquisition GenBank: BC009001
• Symbole du gène: OXCT1
• Identification du gène (NCBI): 5019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OXCT1 (also know as SCOT), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)