Anticorps Monoclonal anti-ORM1/2

ORM1/2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

5F2H7

N° de cat : 66097-1-Ig

Synonymes

AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1

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Galerie de données de validation

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  WB analysis using 66097-1-Ig

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis using 66097-1-Ig

  Untreated and PNGase F-treated lysates of HuH-7 cells were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis of human plasma using 66097-1-Ig

  0.8μL human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66097-1-Ig

  25μg human testis tissue was subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66097-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66097-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66097-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66097-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66097-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50.

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  IHC staining of human liver cancer using 66097-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50.

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  IF Staining of HepG2 using 66097-1-Ig

  Immunofluorescent analysis of () fixed HepG2 cells using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:25.

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  FC experiment of HepG2 using 66097-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ORM1/2 (66097-1-Ig, Clone:5F2H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules HuH-7, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66097-1-Ig cible ORM1/2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ORM1/2 Protéine recombinante Ag19248
• Nom complet: orosomucoid 1
• Masse moléculaire calculée: 201 aa, 24 kDa
• Poids moléculaire observé: 40-47 kDa
• Numéro d’acquisition GenBank: BC026238
• Symbole du gène: ORM1
• Identification du gène (NCBI): 5004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.

Publications

Species	Application	Title
human	WB	Bioengineered; Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9.; Authors - Luo Qiong

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C