Anticorps Monoclonal anti-OPA1

• Phare
• Validé par KD/KO

OPA1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

CloneNo.

1B2D8

N° de cat : 66583-1-Ig

Synonymes

KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

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Galerie de données de validation

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  WB analysis using 66583-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66583-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66583-1-Ig (OPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66583-1-Ig

  WB result of OPA1 antibody (66583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells.

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  IHC staining of mouse brain using 66583-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66583-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66583-1-Ig (OPA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Y79, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IF: See 1 publications below

Informations sur le produit

66583-1-Ig cible OPA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris, fish
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OPA1 Protéine recombinante Ag26868
• Nom complet: optic atrophy 1 (autosomal dominant)
• Masse moléculaire calculée: 960 aa, 112 kDa
• Poids moléculaire observé: 100 kDa and 80-90 kDa
• Numéro d’acquisition GenBank: BC075805
• Symbole du gène: OPA1
• Identification du gène (NCBI): 4976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Publications

Species	Application	Title
mouse	WB	J Endocrinol; ChREBP-β regulates thermogenesis in brown adipose tissue.; Authors - Chunchun Wei
fish	WB	J Physiol Sci; Cardioprotective responses to aerobic exercise-induced physiological hypertrophy in zebrafish heart; Authors - Zhanglin Chen
	WB	Behav Brain Res; Protective effect of exercise training against the progression of Alzheimer's disease in 3xTg-AD mice.; Authors - Donghyun Kim
mouse	WB	Eur J Pharmacol; Dihydromyricetin contributes to weight loss via pro-browning mediated by mitochondrial fission in white adipose; Authors - Xiaowei Xiong
human	WB	Cell Death Dis; Cadmium-induced apoptosis of Leydig cells is mediated by excessive mitochondrial fission and inhibition of mitophagy; Authors - Lingna Yi
rat	WB	Aging (Albany NY); Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF; Authors - Xiao-Lin Jiang