Anticorps Polyclonal de lapin anti-OPA1

• Phare
• Validé par KD/KO

OPA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (7)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

103

Avis (1)

N° de cat : 27733-1-AP

Synonymes

KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

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Galerie de données de validation

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  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27733-1-AP

  WB result of OPA1 antibody (27733-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 27733-1-AP

  IP result of anti-OPA1(IP:27733-1-AP, 4ug; Detection:27733-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 98 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

27733-1-AP cible OPA1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, poisson-zèbre, porc, poulet, souris, Hamster, duck
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OPA1 Protéine recombinante Ag26887
• Nom complet: optic atrophy 1 (autosomal dominant)
• Masse moléculaire calculée: 960 aa, 112 kDa
• Poids moléculaire observé: 100 kDa, 80-90 kDa
• Numéro d’acquisition GenBank: BC075805
• Symbole du gène: OPA1
• Identification du gène (NCBI): 4976
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Publications

Species	Application	Title
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
chicken	WB	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong
human	WB	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (02-21-2023)

Works well in human adipocyte cells, we got clean bands at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes