Anticorps Polyclonal de lapin anti-ODC1

ODC1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 28728-1-AP

Synonymes

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

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Galerie de données de validation

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  WB analysis using 28728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28728-1-AP (ODC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 28728-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 28728-1-AP (ODC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of LNCaP using 28728-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using ODC1 antibody (28728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules LNCaP, tissu de thymus de souris
• Résultats positifs en IF: cellules LNCaP,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

28728-1-AP cible ODC1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ODC1 Protéine recombinante Ag29751
• Nom complet: ornithine decarboxylase 1
• Masse moléculaire calculée: 461 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC025296
• Symbole du gène: ODC1
• Identification du gène (NCBI): 4953
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

Publications

Species	Application	Title
human	WB	Mol Ther; Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer.; Authors - Daryl Jin Tai Tay
human	WB	Front Oncol; CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in Neuroblastoma.; Authors - Lin Qiu
human	IHC	Hum Mol Genet; Ornithine Decarboxylase, the rate-limiting enzyme of polyamine synthesis, modifies brain pathology in a mouse model of tuberous sclerosis complex.; Authors - David Kapfhamer
mouse	WB	Biochim Biophys Acta Mol Cell Res; Urea transporter B downregulates polyamines levels in melanoma B16 cells via p53 activation.; Authors - Jiajing Li
human	WB	Onco Targets Ther; Simultaneous Inhibition of Ornithine Decarboxylase 1 and Pyruvate Kinase M2 Exerts Synergistic Effects Against Hepatocellular Carcinoma Cells.; Authors - Zhirui Zeng
pig	WB	Vet Microbiol; Polyamine regulation of porcine reproductive and respiratory syndrome virus infection depends on spermidine-spermine acetyltransferase 1.; Authors - Yanrong Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (09-07-2023)

Works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 or 1:2000
• Cell Tissue Type: Hela, HepG2, mouse liver, mouse brain

ODC1 Antibody Western Blot validation (1:1000 or 1:2000 dilution) in Hela, HepG2, mouse liver, mouse brain (Cat no:28728-1-AP)

FH

Janelle (Verified Customer) (09-12-2022)

Highly specific to target, clean blots

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000