Anticorps Monoclonal anti-OCIAD1

• Phare
• Validé par KD/KO

OCIAD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1C10C3

N° de cat : 66698-1-Ig

Synonymes

Asrij, FLJ20455, OCIA, OCIA domain containing 1, OCIAD1, TPA018

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Galerie de données de validation

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  WB analysis of HeLa using 66698-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 66698-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 66698-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 66698-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 66698-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 66698-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 66698-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la thyroïde humain, tissu de cancer du pancréas humain, tissu rénal humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du foie humain, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

66698-1-Ig cible OCIAD1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: OCIAD1 Protéine recombinante Ag9977
• Nom complet: OCIA domain containing 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC003409
• Symbole du gène: OCIAD1
• Identification du gène (NCBI): 54940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).

Publications

Species	Application	Title
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur ; KD Validated