Anticorps Polyclonal de lapin anti-Nlrp3

Nlrp3 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 30109-1-AP

Synonymes

AGTAVPRL, AII/AVP, Cias1, FCAS, FCU, Mmig1, MWS, NALP3, Nlrp3, Pypaf1

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Galerie de données de validation

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  WB analysis using 30109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  Non-treated RAW 264.7 cells and LPS-treated RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  rat spleen tissue was subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 30109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Nlrp3 antibody (30109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: J774A.1 cells, cellules RAW 264.7, cellules RAW 264.7 traitées au LPS, cellules THP-1, tissu splénique de rat
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

30109-1-AP cible Nlrp3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nlrp3 Protéine recombinante Ag32515
• Nom complet: NLR family, pyrin domain containing 3
• Masse moléculaire calculée: 118kd
• Poids moléculaire observé: 108-120 kDa
• Numéro d’acquisition GenBank: NM_145827
• Symbole du gène: Nlrp3
• Identification du gène (NCBI): 216799
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse Nlrp3.

Publications

Species	Application	Title
human	WB	JACC Basic Transl Sci; PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia; Authors - Meixin Zhang