Anticorps Monoclonal anti-NeuN

NeuN Monoclonal Antibody for FC, IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

66

CloneNo.

3A4C1

Avis (4)

N° de cat : 66836-1-Ig

Synonymes

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

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Galerie de données de validation

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  IHC staining of rat cerebellum using 66836-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of rat cerebellum using 66836-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IF Staining of mouse cerebellum using 66836-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green).

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  IF Staining of rat cerebellum using 66836-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green).

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  FC experiment of SH-SY5Y using 66836-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NeuN (66836-1-Ig, Clone:3A4C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IHC: tissu de cervelet de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cervelet de souris, tissu de cervelet de rat
• Résultats positifs en cytométrie: cellules SH-SY5Y,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 4 publications below
• IF: See 58 publications below

Informations sur le produit

66836-1-Ig cible NeuN dans les applications de IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NeuN Protéine recombinante Ag28016
• Nom complet: hexaribonucleotide binding protein 3
• Numéro d’acquisition GenBank: NM_001082575
• Symbole du gène: hCG_1776007
• Identification du gène (NCBI): 146713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.

Publications

Species	Application	Title
rat	IF	Microbiome; The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.; Authors - Weiping Xiao
mouse	IF	Cell Death Dis; Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy; Authors - Yang Deng
rat	IF	Cell Death Dis; ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis; Authors - Yaxuan Zhang
mouse	IF	Environ Int; Polystyrene micro- and nano-particle coexposure injures fetal thalamus by inducing ROS-mediated cell apoptosis.; Authors - Diqi Yang
mouse	IF	Elife; Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain; Authors - Shahzad S Khan
rat	IF	Antioxidants (Basel); Epimedium Aqueous Extract Ameliorates Cerebral Ischemia/Reperfusion Injury through Inhibiting ROS/NLRP3-Mediated Pyroptosis; Authors - Xiaoyu Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

This monoclonal NeuN worked well for mouse tissues.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Retina

FH

Silvia (Verified Customer) (08-11-2022)

it works well for Immunofluorescence on mature neurons

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: hiPSCs-derived neurons

FH

Delphine (Verified Customer) (07-25-2022)

Immunohistochemistry with the NeuN antibody on frozen spinal cord worked but the labeling must be optimized because there is a lot of background noise.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: tissue

FH

q (Verified Customer) (01-05-2022)

It is OK to use it in WB, but several non-specific bands above the expected MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

NeuN Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66836-1-Ig)

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011