Anticorps Monoclonal anti-NUMB

• Phare
• Validé par KD/KO

NUMB Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

2G12B7

N° de cat : 60137-1-Ig

Synonymes

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

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Galerie de données de validation

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  WB analysis of human brain using 60137-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 60137-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 60137-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60137-1-Ig (NUMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 60137-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60137-1-Ig (NUMB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 60137-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60137-1-Ig (NUMB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 60137-1-Ig

  Immunofluorescent analysis of A431 cells using 60137-1-Ig (NUMB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain,
• Résultats positifs en IHC: tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

60137-1-Ig cible NUMB dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: numb homolog (Drosophila)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: BC068476
• Symbole du gène: NUMB
• Identification du gène (NCBI): 8650
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.

Publications

Species	Application	Title
rat	WB,IF	Sci Rep; Hepatic stem cell Numb gene is a potential target of Huang Qi Decoction against cholestatic liver fibrosis.; Authors - Wen Xu ; KD Validated
mouse	WB,IF,CoIP	Neurosci Lett; Numb deficiency causes impaired trafficking of mGlu5 in neurons and autistic-like behaviors.; Authors - Na Wang ; KO Validated
human	WB	Mol Biol Rep; Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5.; Authors - Na Wang
rat	WB	Stem Cell Res Ther; BM-MSCs overexpressing the Numb enhance the therapeutic effect on cholestatic liver fibrosis by inhibiting the ductular reaction; Authors - Yan-Nan Xu
human	IF	Cell Rep; Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas; Authors - Nick A Kuburich