Anticorps Monoclonal anti-NUFIP2

NUFIP2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A4E3

Avis (1)

N° de cat : 67195-1-Ig

Synonymes

182 FIP, 82 FIP, FIP 82, FLJ10976, FMRP interacting protein 2, KIAA1321, NUFIP2, PIG1

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Galerie de données de validation

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  WB analysis using 67195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67195-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67195-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67195-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67195-1-Ig cible NUFIP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NUFIP2 Protéine recombinante Ag29040
• Nom complet: nuclear fragile X mental retardation protein interacting protein 2
• Masse moléculaire calculée: 695 aa, 76 kDa
• Poids moléculaire observé: 76 kDa, 82 kDa
• Numéro d’acquisition GenBank: BC108307
• Symbole du gène: NUFIP2
• Identification du gène (NCBI): 57532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

An excellent antibody with great sensitivity and specificity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T