Anticorps Monoclonal anti-NSE

NSE Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6F8G3

N° de cat : CL488-66150

Synonymes

ENO2, Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE

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Galerie de données de validation

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  IF Staining of mouse brain using CL488-66150

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66150 (NSE antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL488-66150

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66150 (NSE antibody) at dilution of 1:50.

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  FC experiment of neuronal cells derived from human dental pulp stem using CL488-66150

  Neuronal cells derived from human dental pulp stem cells were fixed in 70% ethanol and blocked in 0.1% BSA in Staining Buffer, then stained with CoraLite® Plus 488-conjugated NSE Monoclonal Antibody (CL488-66150) (red) or unstained (blue). Image credit: @stemcell_art on Instagram

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  FC experiment of SH-SY5Y using CL488-66150

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NSE (CL488-66150, Clone:6F8G3) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66150 cible NSE dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NSE Protéine recombinante Ag19106
• Nom complet: enolase 2 (gamma, neuronal)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC002745
• Symbole du gène: ENO2
• Identification du gène (NCBI): 2026
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. And this antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C