Anticorps Monoclonal anti-NRF2, NFE2L2

• Phare
• Validé par KD/KO

NRF2, NFE2L2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

104

CloneNo.

1E9E3

Avis (1)

N° de cat : 66504-1-Ig

Synonymes

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2, NRF2, NFE2L2

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Galerie de données de validation

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  WB analysis of HeLa using 66504-1-Ig

  Untreated and MG132 treated Hela cells were subjected to SDS PAGE followed by western blot with 66504-1-Ig (NRF2, NFE2L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 96 publications below
• IHC: See 8 publications below
• IF: See 20 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

66504-1-Ig cible NRF2, NFE2L2 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NRF2, NFE2L2 Protéine recombinante Ag9469
• Nom complet: nuclear factor (erythroid-derived 2)-like 2
• Masse moléculaire calculée: 605 aa, 68 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC011558
• Symbole du gène: NFE2L2
• Identification du gène (NCBI): 4780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Publications

Species	Application	Title
human	WB,IHC,IP	J Clin Invest; FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway; Authors - Yunjiang Zhou
mouse	CoIP	Nat Commun; Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection.; Authors - Yao Wei
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang
mouse,human	WB,IF	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB,IHC,IF	Phytomedicine; Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib; Authors - Chen Yang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In tissues, we observed bands at varying positions, but in cancer cell lines, the bands appeared significantly clearer. The optimal concentration was found to be 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer cell lines

NRF2, NFE2L2 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:66504-1-Ig)