Anticorps Monoclonal anti-NR1H3

NR1H3 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

4A4F7

N° de cat : 60134-1-Ig

Synonymes

Liver X receptor alpha, LXR a, LXRA, NR1H3, Oxysterols receptor LXR alpha, RLD 1

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Galerie de données de validation

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  WB analysis using 60134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60134-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60134-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60134-1-Ig (NR1H3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 60134-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60134-1-Ig (NR1H3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules T-47D, cellules COLO 320, cellules HepG2, cellules MCF-7
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

60134-1-Ig cible NR1H3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NR1H3 Protéine recombinante Ag6184
• Nom complet: nuclear receptor subfamily 1, group H, member 3
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa 44 kDa
• Numéro d’acquisition GenBank: BC041172
• Symbole du gène: NR1H3
• Identification du gène (NCBI): 10062
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR1H3, also known as LXRA, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It's also a ligand-activated LXRs and forms heterodimers with retinoid X receptor, then participates in regulating expression of target genes containing LXR response elements.

Publications

Species	Application	Title
mouse	WB	Food Funct; CD36 and DGAT2 facilitate the lipid-lowering effect of chitooligosaccharides via fatty acid intake and triglyceride synthesis signaling.; Authors - Xin Shen
human	IHC	J Transl Med; Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer; Authors - Wei Tang