Anticorps Monoclonal anti-NR1H2

• Phare
• Validé par KD/KO

NR1H2 Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

10

CloneNo.

1E12B10

Avis (1)

N° de cat : 60345-1-Ig

Synonymes

Liver X receptor beta, LXR b, LXRB, NER, NER I, NR1H2, Nuclear receptor NER, Oxysterols receptor LXR beta, RIP15, UNR

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Galerie de données de validation

• 

  WB analysis of human liver using 60345-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60345-1-Ig (NR1H2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 60345-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60345-1-Ig (NR1H2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 60345-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NR1H2 (60345-1-Ig, Clone:1E12B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, tissu hépatique de porc
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

60345-1-Ig cible NR1H2 dans les applications de WB, IHC, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NR1H2 Protéine recombinante Ag5635
• Nom complet: nuclear receptor subfamily 1, group H, member 2
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC047750
• Symbole du gène: NR1H2
• Identification du gène (NCBI): 7376
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NR1H2, also known as LXRB, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It preferentially bind to double-stranded oligonucleotide direct repeats, which have the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). It involves in the regulation of cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

Publications

Species	Application	Title
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
human	WB	Front Oncol; EGFR/FOXO3A/LXR-α Axis Promotes Prostate Cancer Proliferation and Metastasis and Dual-Targeting LXR-α/EGFR Shows Synthetic Lethality.; Authors - Tingting Chen
human	WB	J Lipid Res; 25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism.; Authors - Ying Liu ; KD Validated
human	WB	J Lipid Res; Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.; Authors - Ying Liu ; KO Validated
mouse,human	WB	J Biol Chem; TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells.; Authors - Dan Zhao
mouse,human	WB	J Agric Food Chem; Diosmetin Ameliorates Nonalcoholic Steatohepatitis through Modulating Lipogenesis and Inflammatory Response in a STAT1/CXCL10-Dependent Manner.; Authors - Nanxuan Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zehua (Verified Customer) (11-07-2019)

This antibody works well in liver lysates from wild-type and hLXR knockin mouse.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

NR1H2 Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (Cat no:60345-1-Ig)