Anticorps Monoclonal anti-NOX4

NOX4 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

4E5F1

N° de cat : 67681-1-Ig

Synonymes

Kidney oxidase 1, KOX, KOX 1, NADPH oxidase 4, NOX4, Renal NAD(P)H oxidase, RENOX

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Galerie de données de validation

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  WB analysis using 67681-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67681-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 67681-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67681-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 67681-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67681-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 67681-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HUVEC using 67681-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MG U-87
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67681-1-Ig cible NOX4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NOX4 Protéine recombinante Ag6176
• Nom complet: NADPH oxidase 4
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC040105
• Symbole du gène: NOX4
• Identification du gène (NCBI): 50507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage (PMID: 11728818, PMID: 29285262, PMID: 14670934). Nox4 in cardiac myocytes is primarily expressed in mitochondria, and upregulation of Nox4 induced by hypertrophic stimuli elicits mitochondrial dysfunction and cardiac failure. In breast or ovarian tumor cells, mitochondrial Nox4 contributes to oncogenesis. In vascular endothelial cells, however, Nox4 is expressed in the endoplasmic reticulum (ER) and plays a specific role in redox-mediated ER signaling (PMID: 24259511).

Publications

Species	Application	Title
human	WB	Biomedicines; Identification of NOX4 as a New Biomarker in Hepatocellular Carcinoma and Its Effect on Sorafenib Therapy; Authors - Hui-Zhou Li
human	IF	Antioxidants (Basel); Sirtuin1 Mediates the Protective Effects of Echinacoside against Sepsis-Induced Acute Lung Injury via Regulating the NOX4-Nrf2 Axis; Authors - Weixi Xie
rat	WB	Antioxidants (Basel); The Effect of Tauroursodeoxycholic Acid (TUDCA) Treatment on Pregnancy Outcomes and Vascular Function in a Rat Model of Advanced Maternal Age; Authors - Mazhar Pasha