Anticorps Polyclonal de lapin anti-eNOS

eNOS Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

33

N° de cat : 27120-1-AP

Synonymes

cNOS, Constitutive NOS, EC NOS, ECNOS, Endothelial NOS, eNOS, NOS type III, NOS3, NOSIII

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Galerie de données de validation

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  WB analysis using 27120-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27120-1-AP (NOS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27120-1-AP

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 27120-1-AP (eNOS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules bEnd.3, tissu rénal de rat, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 30 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

27120-1-AP cible eNOS dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: eNOS Protéine recombinante Ag25712
• Nom complet: nitric oxide synthase 3 (endothelial cell)
• Poids moléculaire observé: 133 kDa, 69 kDa
• Symbole du gène: NOS3
• Identification du gène (NCBI): 4846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.

Publications

Species	Application	Title
mouse	WB	Redox Biol; Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy; Authors - Lijie Ma
mouse	WB	Redox Biol; Non-steroidal mineralocorticoid receptor antagonist finerenone ameliorates mitochondrial dysfunction via PI3K/Akt/eNOS signaling pathway in diabetic tubulopathy; Authors - Lan Yao
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
human	WB	Int J Biol Macromol; Selection of goat β-casein derived ACE-inhibitory peptide SQPK and insights into its effect and regulatory mechanism on the function of endothelial cells; Authors - Yulong Wu
rat	WB	Oxid Med Cell Longev; Tissue Kallikrein Protects Rat Prostate against the Inflammatory Damage in a Chronic Autoimmune Prostatitis Model via Restoring Endothelial Function in a Bradykinin Receptor B2-Dependent Way.; Authors - Mengyang Zhang
mouse	WB,IHC	Food Funct; Procyanidins extracted from the litchi pericarp ameliorate atherosclerosis in ApoE knockout mice: their effects on nitric oxide bioavailability and oxidative stress; Authors - Shuang Rong