Anticorps Monoclonal anti-NEIL1

NEIL1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C6D6

N° de cat : CL488-67012

Synonymes

DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FLJ22402, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-67012

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated NEIL1 antibody (CL488-67012, Clone: 1C6D6 ) at dilution of 1:100.

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Applications testées

• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67012 cible NEIL1 dans les applications de IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NEIL1 Protéine recombinante Ag8307
• Nom complet: nei endonuclease VIII-like 1 (E. coli)
• Masse moléculaire calculée: 390 aa, 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC010876
• Symbole du gène: NEIL1
• Identification du gène (NCBI): 79661
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)