Anticorps Monoclonal anti-NDUFS5

NDUFS5 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2C2A5

N° de cat : 66053-1-Ig

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 66053-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66053-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66053-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66053-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66053-1-Ig (NDUFS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66053-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66053-1-Ig(NDUFS5 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules L02, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66053-1-Ig cible NDUFS5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFS5 Protéine recombinante Ag7663
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC001884
• Symbole du gène: NDUFS5
• Identification du gène (NCBI): 4725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS5 gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. The NDUFS5 mRNA is expressed ubiquitously in human tissues, with a relative higher expression in human heart, skeletal muscle, liver, kidney and fetal heart (PMID: 10070614). The NDUFS5 (15 kDa) subunit located at mitochondrion and contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds (PMID: 21310150). Proteinteach’s NDUFS5 antibody 66053-1-Ig is capable of detecting the 15 kDa protein in Western blotting.