Anticorps Polyclonal de lapin anti-NBN / NBS1

• Phare
• Validé par KD/KO

NBN / NBS1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 55025-1-AP

Synonymes

AT V1, AT V2, ATV, NBN, NBS, NBS1, nibrin, P95

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Galerie de données de validation

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  WB analysis of HeLa using 55025-1-AP

  WB result of NBS1 antibody (55025-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NBS1 transfected HeLa cells.

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  WB analysis of HeLa using 55025-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55025-1-AP (NBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 55025-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55025-1-AP (NBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55025-1-AP

  IP result of anti-NBS1(IP:55025-1-AP, 4ug; Detection:55025-1-AP 1:3000) with HeLa cells lysate 1320 ug.

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  IF Staining of HepG2 using 55025-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 55025-1-AP (NBS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 55025-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using 55025-1-AP (NBS1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF: cellules HepG2, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55025-1-AP cible NBN / NBS1 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nibrin
• Masse moléculaire calculée: 85 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: NM_002485
• Symbole du gène: NBN
• Identification du gène (NCBI): 4683
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NBN, also named as NBS, NBS1 and P95, is a component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. Defects in NBN are the cause of Nijmegen breakage syndrome (NBS). Defects in NBN are a cause of genetic susceptibility to breast cancer (BC). Defects in NBN may be associated with aplastic anemia. Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL). The antibody is specific to NBN. The full-length NBN protein, with an apparent molecular weight of 95 kDa and the two protein fragments of 26 and 70 kDa arising from the c.657_661del5 (p.K219fsX19) mutation, and the 80 kDa protein found in patient RR with the mutation c.742_743insGG leading to excision of exons 6 and 7 from the NBN mRNA are shown. (PMID: 26265251) The predicted molecular weight of NBN protein (p95) is 85kDa, actually detection result is about 95kDa(PMID: 23762398).

Publications

Species	Application	Title
human	WB	Mol Cell; C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.; Authors - Yongtai Bai
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB	Nucleic Acids Res; LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA.; Authors - Liping Shen
mouse	WB,IF	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang ; KD Validated
human	WB	Oncogene; TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex; Authors - Ya Wang
rat	WB	Mol Cancer Res; PKCð regulates chromatin remodeling and DNA repair through SIRT6; Authors - Trisiani Affandi