Anticorps Polyclonal de lapin anti-Myosin Light Chain 2/MLC-2V
Myosin Light Chain 2/MLC-2V Polyclonal Antibody for IF
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, poisson-zèbre, rat, souris
Applications
IF
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
N° de cat : CL488-10906
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF | tissu cardiaque de souris, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| IF | See 1 publications below |
Informations sur le produit
CL488-10906 cible Myosin Light Chain 2/MLC-2V dans les applications de IF et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris
| Réactivité | Humain, poisson-zèbre, rat, souris |
| Réactivité citée | Humain |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Myosin Light Chain 2/MLC-2V Protéine recombinante Ag1356 |
| Nom complet | myosin, light chain 2, regulatory, cardiac, slow |
| Masse moléculaire calculée | 19 kDa |
| Poids moléculaire observé | 19 kDa |
| Numéro d’acquisition GenBank | BC015821 |
| Symbole du gène | MYL2 |
| Identification du gène (NCBI) | 4633 |
| Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Background
MYL2 belongs to the myosin family of motor proteins. Their common features are ATP hydrolysis, actin binding, and potential for kinetic energy transduction. MYL2 stands for Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2), also known as the regulatory light chain of myosin (RLC). This isoform is distinct from those expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B), and cardiac atrial muscle (MYL7) (PMID: 21345328). Due to its tissue specificity, MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
What is the molecular weight of MYL2?
There are two isoforms of this protein in humans that differ only slightly in length and produce proteins composed of 152 and 166 aa, which correspond to about 19 kDa (PMID: 16678204).
What is the subcellular localization of MYL2?
It is uniquely expressed in the cytoplasm of heart muscles. It colocalizes with actin cytoskeleton staining.
What is the tissue specificity of MYL2?
MYL2 is specifically expressed in ventricular cardiac tissue.
What is the function of MYL2 in cardiac tissue?
MYL2 is a contractile protein that plays a role in heart development and function. During cardiogenesis it plays an early role in cardiac contractility by promoting cardiac myofibril assembly and represents one of the earliest markers of ventricular specification (PMID 8506363).
In general, MYL2 interacts with the neck/tail region of the muscle thick filament protein myosin to regulate myosin motility and function (PMID 8316858). Its N-terminal domain is responsible for calcium and magnesium binding at their activating concentrations. This induces a functionally important conformational change (PMID: 18202317). However, the ion dissociation rate is not fast enough to modulate cardiac contractility on a beat-by-beat basis (PMIDs: 188447, 8804617). In addition, RLC function can be modulated by posttranslational modifications such as phosphorylation and deamidation in the N-terminal region. As a result, a significant change in the charge of the protein is introduced, which undoubtedly alters its interaction with the C-terminal myosin alpha-helical domain (PMID: 26074085).
What is MYL2's involvement in disease?
Diseases associated with MYL2 dysfunction include forms of familial hypertrophic cardiomyopathies, congenital fiber-type disproportion, and heart failure (PMID: 26074085).
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Myosin Light Chain 2/MLC-2V antibody CL488-10906 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Commun Signal Downregulation of connexin 43-based gap junctions underlies propofol-induced excessive relaxation in hypertensive vascular smooth muscle cells |
