Anticorps Polyclonal de lapin anti-Mecr

Mecr Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 51027-2-AP

Synonymes

AI195831, Mecr, Nrbf1

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Galerie de données de validation

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  WB analysis using 51027-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51027-2-AP (Mecr antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 51027-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 51027-2-AP (Mecr antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 51027-2-AP

  IP Result of anti-Mecr (IP:51027-2-AP, 4ug; Detection:51027-2-AP 1:1500) with mouse heart tissue lysate 4800ug.

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  IHC staining of human ovary tumor using 51027-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51027-2-AP (Mecr antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of C2C12 using 51027-2-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Mecr antibody (51027-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de souris, tissu d'estomac de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IF: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

51027-2-AP cible Mecr dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, levure, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Mecr Protéine recombinante Ag0465
• Nom complet: mitochondrial trans-2-enoyl-CoA reductase
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC003864
• Symbole du gène: Mecr
• Identification du gène (NCBI): 26922
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial Trans-2-Enoyl-CoA Reductase is abbrivated as MECR, involved in human mitochonrial fatty acid synthesis(mtFAS) as an oxidoreductase. (PMID: 27817865) MECR has molecular weight can be calculated around 37KDa. With immunopercipitation reaction on mouse heart samples, the IgG heavy chain, dimer of MECR is around 65KDa. Numerous studies have shown disorder of lipid metabolism is closely related with malignance, especially in liver cancer. As futher research suggests that MECR has close realitionship with cell prolifereation, appotosis, and metastasis (PMID: 31178528)

Publications

Species	Application	Title
human	WB	Am J Hum Genet; MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder.; Authors - Gali Heimer
mouse	WB	Elife; Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria.; Authors - Sara M Nowinski
mouse	IHC,IF	J Neurosci; Impaired mitochondrial fatty acid synthesis leads to neurodegeneration in mice.; Authors - Remya R Nair
mouse	WB	Hum Mol Genet; Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice.; Authors - Remya R Nair
yeast	WB	Nat Commun; An engineered variant of MECR reductase reveals indispensability of long-chain acyl-ACPs for mitochondrial respiration; Authors - M Tanvir Rahman