Anticorps Monoclonal anti-Mcp1

• Phare
• Validé par KD/KO

Mcp1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

souris et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

120

CloneNo.

1B9F7

N° de cat : 66272-1-Ig

Synonymes

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF

---

Galerie de données de validation

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66272-1-Ig

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66272-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66272-1-Ig (Mcp1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66272-1-Ig (Mcp1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66272-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66272-1-Ig (Mcp1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules DC2.4, cellules RAW 264.7 traitées au LPS, tissu cérébral de souris, tissu placentaire de souris, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 90 publications below
• IHC: See 29 publications below
• IF: See 14 publications below
• CoIP: See 1 publications below

Informations sur le produit

66272-1-Ig cible Mcp1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Lapin, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mcp1 Protéine recombinante Ag24085
• Nom complet: chemokine (C-C motif) ligand 2
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM-011333
• Symbole du gène: Ccl2
• Identification du gène (NCBI): 20296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

Publications

Species	Application	Title
	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
mouse	IF	Hepatology; Single-cell transcriptomic analysis reveals a hepatic stellate cell-activation roadmap and myofibroblast origin during liver fibrosis.; Authors - Wu Yang
mouse	IHC	Cell Death Differ; Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis; Authors - Qing-Xin Ji
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
mouse	WB	Nat Commun; Nuclear pore protein NUP210 depletion suppresses metastasis through heterochromatin-mediated disruption of tumor cell mechanical response.; Authors - Ruhul Amin