Anticorps Monoclonal anti-MYPT1

MYPT1 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2A1A9

N° de cat : 66506-1-Ig

Synonymes

MBS, MYPT1, PPP1R12A

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Galerie de données de validation

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  WB analysis of Raji using 66506-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66506-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66506-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66506-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66506-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66506-1-Ig (MYPT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66506-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MYPT1 antibody (66506-1-Ig, Clone: 2A1A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66506-1-Ig cible MYPT1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYPT1 Protéine recombinante Ag17496
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Masse moléculaire calculée: 1030 aa, 115 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC111752
• Symbole du gène: PPP1R12A
• Identification du gène (NCBI): 4659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myosin phosphatase target subunit 1(MYPT1), which is also called PPP1R12A, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization.

Publications

Species	Application	Title
rat	WB	Brain Res; The ROCK inhibitor Y-27632 ameliorates blood-spinal cord barrier disruption by reducing tight junction protein degradation via the MYPT1-MLC2 pathway after spinal cord injury in rats.; Authors - Sheng Chang