Anticorps Monoclonal anti-MYH14

MYH14 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1B4C9

N° de cat : 66825-1-Ig

Synonymes

DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C

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Galerie de données de validation

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  WB analysis of COLO 320 using 66825-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 66825-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 66825-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 66825-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HT-29 using 66825-1-Ig

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human MYH14 (66825-1-Ig, Clone:1B4C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HT-29
• Résultats positifs en IHC: tissu de côlon humain, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris,
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:100000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

66825-1-Ig cible MYH14 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYH14 Protéine recombinante Ag28063
• Nom complet: myosin, heavy chain 14
• Masse moléculaire calculée: 228 kDa
• Poids moléculaire observé: 200-230 kDa
• Numéro d’acquisition GenBank: NM_001077186
• Symbole du gène: MYH14
• Identification du gène (NCBI): 79784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).

Publications

Species	Application	Title
rat	WB,IF	J Cell Sci; Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation; Authors - Helena Sofia Domingues ; KD Validated
	IF	J Cell Sci; Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation.; Authors - Helena Sofia Domingues