Anticorps Monoclonal anti-MYH1

MYH1 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1G10H9

Avis (1)

N° de cat : 67299-1-Ig

Synonymes

MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 67299-1-Ig

  1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig skeletal muscle using 67299-1-Ig

  1 μg pig skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 67299-1-Ig

  1 μg mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 67299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of C2C12 using 67299-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1 (67299-1-Ig, Clone:1G10H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain, tissu de muscle squelettique de porc, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:2000-1:20000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

67299-1-Ig cible MYH1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: porc, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYH1 Protéine recombinante Ag17129
• Nom complet: myosin, heavy chain 1, skeletal muscle, adult
• Masse moléculaire calculée: 1939 aa, 223 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC114545
• Symbole du gène: MYH1
• Identification du gène (NCBI): 4619
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

Publications

Species	Application	Title
pig	WB	Food Funct; Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model.; Authors - Lingyu Zhang
mouse	IF,WB	Int J Mol Sci; Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling Pathway; Authors - Xiaomao Luo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Moon (Verified Customer) (10-10-2022)

normal tissue-1/2 (N1/N2) tumor tissue-1/2/3 (T1/T2/T3)

• Applications: Western Blot
• Primary Antibody Dilution: 1:100000
• Cell Tissue Type: renal carcinoma cells

MYH1 Antibody Western Blot validation (1:100000 dilution) in renal carcinoma cells (Cat no:67299-1-Ig)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C