Anticorps Polyclonal de lapin anti-MUC4

MUC4 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC

Conjugaison

Non conjugué

N° de cat : 28703-1-AP

Synonymes

Ascites sialoglycoprotein, Ascites sialoglycoprotein 1, Ascites sialoglycoprotein 2, ASGP, ASGP 1 Mucin 4 beta chain, ASGP 2, HSA276359, MUC 4, MUC4, Mucin 4, Mucin-4, Testis mucin, Tracheobronchial mucin

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Galerie de données de validation

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  WB analysis using 28703-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28703-1-AP (MUC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 28703-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28703-1-AP (MUC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28703-1-AP cible MUC4 dans les applications de WB, IHC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUC4 Protéine recombinante Ag29799
• Nom complet: mucin 4, cell surface associated
• Masse moléculaire calculée: 231 kDa
• Poids moléculaire observé: 231 kDa, 113 kDa
• Numéro d’acquisition GenBank: NM_018406
• Symbole du gène: MUC4
• Identification du gène (NCBI): 4585
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUC4, also named as HSA276359, ASGP, MUC-4, testis mucin and mucin-4 is a high molecular weight transmembrane mucin that is overexpressed in many carcinomas and is a risk factor associated with a poor prognosis. MUC4 have a key role in the progression of pancreatic cancer. MUC4 helps in the docking of tumor cells on the endothelial surface.