Anticorps Monoclonal anti-mTOR

• Phare
• Validé par KD/KO

mTOR Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, PLA, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

250

CloneNo.

1G11A3

Avis (3)

N° de cat : 66888-1-Ig

Synonymes

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

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Galerie de données de validation

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  WB analysis using 66888-1-Ig

  Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66888-1-Ig (MTOR antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66888-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66888-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66888-1-Ig

  WB result of mTOR antibody (66888-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells.

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  WB analysis of HeLa using 66888-1-Ig

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

• 

  WB analysis of HEK-293 using 66888-1-Ig

  Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

• 

  WB analysis of HEK-293 using 66888-1-Ig

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

• 

  WB analysis of ROS1728 using 66888-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66888-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66888-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66888-1-Ig

  IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug.

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  IP experiment of HeLa using 66888-1-Ig

  IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug.

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  IHC staining of human colon cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66888-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 66888-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NCI-H1299, cellules NIH/3T3, cellules ROS1728, cellules THP-1, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 242 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 4 publications below

Informations sur le produit

66888-1-Ig cible mTOR dans les applications de WB, PLA, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris, Hamster
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: mTOR Protéine recombinante Ag28395
• Nom complet: FK506 binding protein 12-rapamycin associated protein 1
• Masse moléculaire calculée: 289 kDa
• Poids moléculaire observé: 250-289 kDa
• Numéro d’acquisition GenBank: NM_004958
• Symbole du gène: FRAP1
• Identification du gène (NCBI): 2475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).

Publications

Species	Application	Title
human	WB	Gastroenterology; Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis; Authors - Xiao-Mei Yang
human	WB,IP	Mol Cell; Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells; Authors - Lixin He
human	WB	Mol Cancer; circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.; Authors - Xiaomin Li
human	WB	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
mouse	PLA	Nat Commun; Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.; Authors - Xin Zhou
human	WB	J Clin Invest; Up-regulated YB-1 protein promotes glioblastoma growth through an YB-1/CCT4/mLST8/mTOR pathway.; Authors - Jin-Zhu Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

strong band showed up around 200kDa region in HEK293T cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Macarena (Verified Customer) (10-07-2022)

excellent band. Very concentrated.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: cell lines

FH

Rachel (Verified Customer) (05-24-2021)

Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western

• Applications: Western Blot
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: PC3 cells

mTOR Antibody Western Blot validation (1/50 dilution) in PC3 cells (Cat no:66888-1-Ig)