Anticorps Monoclonal anti-MTHFR

MTHFR Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1F7A3

Avis (1)

N° de cat : 66612-1-Ig

Synonymes

MTHFR

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Galerie de données de validation

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  WB analysis of LNCaP using 66612-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66612-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66612-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66612-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66612-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66612-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66612-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MTHFR antibody (66612-1-Ig, Clone: 1F7A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

66612-1-Ig cible MTHFR dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTHFR Protéine recombinante Ag14970
• Nom complet: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Masse moléculaire calculée: 656 aa, 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC053509
• Symbole du gène: MTHFR
• Identification du gène (NCBI): 4524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

5,10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. MTHFR reduces 5,10-methylenetetrahydrofolate (5,10-methyleneTHF) to 5-methylTHF, the primary carbon donor for methionine production from homocysteine. In humans and mice studies, mutations and polymorphisms in MTHFR are associated with impaired spermatogenesis and male infertility. It has been reported that MTHFR is highly phosphorylated under unperturbed conditions and T34 is the priming phosphorylation site. MTHFR gene has two promoters and isoform (70 kDa and 77 kDa). Although the major form of MTHFR is 77kDa in most tissues, a second isoform of 70kDa was observed after western blotting of human fetal liver and porcine liver. (PMID: 24769206, PMID: 34128976, PMID: 18615588)

Publications

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (06-17-2021)

Good antibody, but not work in mouse.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, human liver, mouse liver and mouse brain

MTHFR Antibody Western Blot validation (1:1000 dilution) in HepG2, human liver, mouse liver and mouse brain (Cat no:66612-1-Ig)