Anticorps Polyclonal de lapin anti-MTCO3

MTCO3 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 55082-1-AP

Synonymes

COIII, COXIII, cytochrome c oxidase III, Cytochrome c oxidase subunit 3, MT CO3, MTCO3

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Galerie de données de validation

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  WB analysis using 55082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55082-1-AP (MTCO3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 55082-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 55082-1-AP (MTCO3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 55082-1-AP

  IP Result of anti-MTCO3 (IP:55082-1-AP, 4ug; Detection:55082-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55082-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55082-1-AP (MTCO3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules C2C12, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cervelet de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IF: See 2 publications below

Informations sur le produit

55082-1-AP cible MTCO3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome c oxidase III
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: YP_003024032
• Symbole du gène: COX3
• Identification du gène (NCBI): 4514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).

Publications

Species	Application	Title
mouse	WB	Cell Res; Active RNA interference in mitochondria.; Authors - Kuanxing Gao
mouse	WB	Diabetes; Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.; Authors - Na Tian
mouse	WB	Int J Mol Sci; Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice.; Authors - Olga A Averina
human	WB	J Med Genet; COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.; Authors - Anabel Martinez Lyons
mouse	IF	Sci China Life Sci; Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival.; Authors - Jialiang Yang
human	WB	Carcinogenesis; Mitochondrial miR-181a-5p promotes glucose metabolism reprogramming in liver cancer by regulating the electron transport chain.; Authors - Xiang Zhuang