Anticorps Monoclonal anti-MTA2

• Phare
• Validé par KD/KO

MTA2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

2G12G4

N° de cat : 66195-1-Ig

Synonymes

Metastasis associated 1 like 1, MTA1 L1 protein, MTA1L1, MTA2, PID

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Galerie de données de validation

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  WB analysis using 66195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66195-1-Ig (MTA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 66195-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66195-1-Ig (MTA2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66195-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTA2 antibody (66195-1-Ig, Clone: 2G12G4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66195-1-Ig

  1X10^6 HepG2 cells were stained with .2ug MTA2 antibody (66195-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules SW480, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, tissu pulmonaire de souris, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:125-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

66195-1-Ig cible MTA2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTA2 Protéine recombinante Ag11713
• Nom complet: metastasis associated 1 family, member 2
• Masse moléculaire calculée: 668 aa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC053650
• Symbole du gène: MTA2
• Identification du gène (NCBI): 9219
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Metastasis-associated protein MTA2, also named as MTA1L1 or PID, is a 668 amino acid protein, which contains one BAH domain, one ELM2 domain, one GATA-type zinc finger and one SANT domain. MTA2 localizes in the nucleus and is widely expressed in many tissues. MTA2 may be involved in the regulation of gene expression as repressor and activator.

Publications

Species	Application	Title
human	WB,IF	Theranostics; LncRNA-MTA2TR functions as a promoter in pancreatic cancer via driving deacetylation-dependent accumulation of HIF-1α.; Authors - Zhu Zeng ; KD Validated
human	WB	Oncol Lett; Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells.; Authors - Shanshan Li
human	WB	Cancers (Basel); ZEB2/TWIST1/PRMT5/NuRD Multicomplex Contributes to the Epigenetic Regulation of EMT and Metastasis in Colorectal Carcinoma; Authors - Yayuan Zheng
human	WB	Nat Chem Biol; ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism; Authors - Linchong Sun ; KD Validated
human	WB	Cell Chem Biol; HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation; Authors - Yufeng Xiao