Anticorps Monoclonal anti-MSH6

• Phare
• Validé par KD/KO

MSH6 Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

2E10B2

N° de cat : 66172-1-Ig

Synonymes

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160

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Galerie de données de validation

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  WB analysis of HEK-293 using 66172-1-Ig

  WB result of MSH6 antibody (66172-1-Ig, 1:1000) with si-Control and si-MSH6 transfected HEK293 cells.

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  WB analysis of HEK-293 using 66172-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66172-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66172-1-Ig

  IP Result of anti-MSH6 (IP:66172-1-Ig, 4ug; Detection:66172-1-Ig 1:600) with HEK-293 cells lysate 1800ug.

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  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66172-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66172-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66172-1-Ig(MSH6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HEK-293 using 66172-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human MSH6 (66172-1-Ig, Clone:2E10B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7, cellules U2OS
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 4 publications below

Informations sur le produit

66172-1-Ig cible MSH6 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MSH6 Protéine recombinante Ag12645
• Nom complet: mutS homolog 6 (E. coli)
• Masse moléculaire calculée: 153 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC004246
• Symbole du gène: MSH6
• Identification du gène (NCBI): 2956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Publications

Species	Application	Title
mouse	WB	J Med Chem; CJ2: A Novel Potent Platinum(IV) Prodrug Enhances Chemo-Immunotherapy by Facilitating PD-L1 Degradation in the Cytoplasm and Cytomembrane; Authors - Renming Fan
human	IHC	Surgery; Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.; Authors - Dingkong Liang
human	IHC	J Surg Oncol; Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.; Authors - Jun Zhu
human	IHC	Neuropathology; Sebaceous adenoma occurring within an intracranial dermoid cyst.; Authors - Takashi Minamisaka
human	IHC	Oncol Lett; HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma.; Authors - Xiangxiang Tao