Anticorps Monoclonal anti-MRPS27

MRPS27 Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1B1B3

N° de cat : 66724-1-Ig

Synonymes

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt

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Galerie de données de validation

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  WB analysis of LNCaP using 66724-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66724-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66724-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66724-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66724-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66724-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A431, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66724-1-Ig cible MRPS27 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MRPS27 Protéine recombinante Ag11047
• Nom complet: mitochondrial ribosomal protein S27
• Masse moléculaire calculée: 168 aa, 19 kDa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC030521
• Symbole du gène: MRPS27
• Identification du gène (NCBI): 23107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Publications

Species	Application	Title
human		Aging (Albany NY); Qualitative and quantitative alterations in intracellular and membrane glycoproteins maintain the balance between cellular senescence and human aging.; Authors - Yoko Itakura
human	WB	Biomedicines; Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema; Authors - Loukmane Karim