Anticorps Monoclonal anti-MPO

• Phare
• Validé par KD/KO

MPO Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgA

Réactivité testée

Humain, rat

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C11F6

Avis (1)

N° de cat : CL488-66177

Synonymes

MPO, myeloperoxidase

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL488-66177

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 MPO antibody (CL488-66177, Clone: 4C11F6 ) at dilution of 1:200.

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  FC experiment of HL-60 using CL488-66177

  1X10^6 HL-60 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MPO (CL488-66177, Clone:4C11F6) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu d'amygdalite humain,
• Résultats positifs en cytométrie: cellules HL-60

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66177 cible MPO dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgA
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MPO Protéine recombinante Ag17564
• Nom complet: myeloperoxidase
• Masse moléculaire calculée: 745 aa, 84 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC130476
• Symbole du gène: MPO
• Identification du gène (NCBI): 4353
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sonja (Verified Customer) (08-04-2023)

The antibody works absolutely perfect in human FFPE tissue. The fluorescence intensity was very strong.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: FFPE Human Tonsil

CL Plus 488 MPO Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in FFPE Human Tonsil (Cat no:CL488-66177)